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PCR primers ended up designed to examination right integration with the five′ and three′ flanks from the drug resistance markers used and also existence of t
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The construction of your unliganded double mutant HIV-1 RT showed that Lys103Asn mutation facilitates coordination of the sodium ion with Lys101 O, Asn103 N and O(delta1), Tyr188 O(eta), and two drinking water molecules. The formation of the binding pocket calls for the elimination of the sodium ion